Polymerase Chain Reaction using “V” Shape Thermal Cycling Program

Convectively driven polymerase chain reaction thermal cycler.

We have fabricated a low-cost disposable polymerase chain reaction thermal chamber that uses buoyancy forces to move the sample solution between the different temperatures necessary for amplification. Three-dimensional, unsteady finite element modeling and a simpler 1-D steady-state model are used together with digital particle image velocimetry data to characterize the flow within the device. ...

Detection of Babesia ovis Using Polymerase Chain Reaction.

On-chip Device for Isothermal, Chemical Cycling Polymerase Chain Reaction

We demonstrate a novel chemical cycling polymerase chain reaction (ccPCR) technique for DNA amplification in a fully working device where temperature is held constant in space and time. We demonstrate successful ccPCR amplification while simultaneously focusing products via isotachophoresis (ITP) for identification of the environmental bacteria E. Coli. We electrophoretically drive the DNA samp...

Failure Prediction of Polymerase Chain Reaction Thermal Cycler

In this paper, we predict the aging of the PCR thermocycler by analyzing the heating and cooling rates of the PCR thermocycler. Two kinds of methods were applied to calculate the rise times of the heating and cooling protocol sections that were inversely related to the heating or cooling rates. The temporal changes of the rise times were investigated over several months. For three thermal cycle...

Solar thermal polymerase chain reaction for smartphone-assisted molecular diagnostics

Nucleic acid-based diagnostic techniques such as polymerase chain reaction (PCR) are used extensively in medical diagnostics due to their high sensitivity, specificity and quantification capability. In settings with limited infrastructure and unreliable electricity, however, access to such devices is often limited due to the highly specialized and energy-intensive nature of the thermal cycling ...